Background: It can be defined that enamel development defects are alterations during the formation of dental enamel, depending on etiological factors that patients usually present, whether due to genetics, systemic diseases, or drug use. Based on the above, validation of classification methods, reliability and utility is required. The Microabrasion and Enamel Teeth Whitening techniques are alternative treatments for dental aesthetics in those cases where demarcated opacities are to be eliminated quickly, effectively and conservatively, for the aesthetic improvement of the patient. Objective: To determine the effectiveness of three treatments for enamel development defects (demarcated opacities) in patients attending the dental clinic of the Universidad Cooperativa de Colombia campus Villavicencio. Method: Experimental study with before and after measurements, a convenience sampling of 16 patients was carried out, where they met the inclusion and exclusion criteria, where they signed informed consent, frequency tables were used to evaluate the results. This study was approved by the University Bioethics Committee. Results: After using the techniques of microabrasion, tooth whitening and the combined technique, color changes were determined with the different techniques used without repercussion on tooth mineralization and post-treatment sensitivity. Conclusion: Although the applications of the different treatments improved the appearance of the demarcated opacities, it was not possible to determine which was the most effective treatment due to the size of the sample and the type of defect, so it is suggested to expand the sample for future studies.