Ehrlichia Canis is an etiological agent of Canine Monocytic Ehrlichiosis, a disease frequently detected in small animals clinic. Zoonotic transmission risk exists where dogs be host of vector tick (Rhipicephalus sanguineus) that transmit E. canis to humans. Objective: To determine the prevalence of E. canis domestic canines by immunoassay (seropositivity) in Villavicencio Meta (Colombia), and associated risk factors to seropositivity. Materials and Methods: convenience sampling were carried out on databases of a veterinary clinic. Sampling criteria were clinical records of clinical signs of canine Ehrlichiosis and results of SNAP 4Dx® immunoassay test (IDEXX Laboratories USA) for antibodies (seropositivity). Thrombocytopenia (<160.k pat/µl) and risk factors as breed, size, sex and age also were considered. Prevalence was established by E. canis presence related to previously mentioned variables. Data were analysed by independence chi square test in EPIDAT3.1® with a confidence level of 95%. Results: 229 clinical records were analysed, and a total prevalence of 63.8% of E. canis were reported. Also breed prevalence were for small 21.4%, for median 24.4% and for large 17.9% (P=0.51). When sex were evaluated, 35.8% males and 27.9% females (P=0.64) were found, as well as for age where <1 year 5.2%, 1-3 years 34.5% and >3 years 24.1% (P=0.01) also were reported. Thrombocytopenia relation with seropositivity show concordance of 38.8%, and 24.8% of discordance (P=0.001). Conclusions: Age over from 1 year and thrombocytopenia are statistically related with seropositivity, however breed and sex no present this relation with the test.