The buffalo species is important to the economies of different countries. It is considered a species of late sexual maturity. In addition, it presents long periods of postpartum anestrus, and the reproductive characteristics are not so good, which causes low litter sizes. Due to that, protocols for the synchronization of estrus are developed to be able to manipulate or control the estrous cycle and sometimes the timing of ovulation. The main purpose of this review was to compare the synchronization protocols published in buffaloes (Bubalus bubalis) through a systematic method using meta-analysis tools. Scientific papers published between 2018 and 2022 about the protocols of estrus synchronization in buffaloes were selected. Papers written in languages other than English, Spanish, and Portuguese were excluded. At the same time, papers with different content for heat synchronization protocols were kept out. Finally, 120 papers from four databases were selected, and 40 of those met the inclusion criteria. Effect diagrams were shown in Forest plot graphics where information was collected on response to estrus, ovulation percentage, percentage of silent estrus, conception rate, and pregnancy rate. Literature-reported reproductive parameters were compared using CI 95%, assuming a normal distribution of the parameters. The standard error was calculated for papers without one reported using the standard deviation and the number of individuals in each paper. The above was reported in forest plot graphics to facilitate the comparison. In conclusion, the Ovsynch protocol, the double application protocol of PgF2a, and the intravaginal device protocol were the synchronization protocols of estrus with the best results in terms of estrus response, ovulation rate, conception rate, and pregnancy rate. Finally, the Ovsynch protocol has been the most effective, given that it uses two important hormones in the estrous cycle, which are GnRH and PgF2a.