The use of prebiotic, probiotic and synbiotics additives has been of great interest due to the increase in restriction of growth promoters caused by bacterial resistance obtained in recent years. A study will be carried out on 240 Hy Line Brown laying hens. The design will be completely randomized (CRD). 8 groups will be randomly distributed, each with 10 repetitions where growth-promoting antibiotics in the diet will be replaced by different levels of prebiotics in combination with probiotics. They will be given 110g of food daily with the following additives VitaCAN, Poultrystar, Vitasym DFM and Enramycin 8% with different inclusion and combination units, obtaining 8 treatments where samples of the intestinal contents will be taken and taken to the microbiology laboratory to perform cultures and antibiogram. by analyzing the bacterial growth and resistance Escherichia coli spp and klebsiella spp in laying hens; The results obtained in the study show that Diet H (Oligosaccharides and Bacillus subtilis) presented the lowest percentage of growth of 10% Klebsiella spp and did not present growth of Escherichia coli spp, on the other hand, the antibiotics with the greatest resistance to the bacteria analyzed in the study. study was ampicillin sulbactam and tetracyclines, finally the use of prebiotics, probiotics and synbiotics allows us to obtain better results in the immunity of animals, it also allows us not to use growth promoters in poultry production taking into account that better results are obtained and We contribute to reducing bacterial resistance.