In order to improve production parameters and business profitability, livestock has implemented innovative technological advances. These advances consider the genotypic and phenotypic characteristics desired in the productive system. In Colombia, due to its varied tropical climate, different technologies are implemented which are used to increase and improve production, taking into account all those characteristics that are essential for the area to work. After choosing the characteristics that have to be improved and choosing the copy that is to be implemented, the subject of sex is reached. For example, for the producers of milk, the birth of males is not desirable, as well as for the meat farms, it is not the birth of the females. However, the outcome of pregnancy is random and with uncertain probabilities of the birth of a male or female. To obtain animals of a specific sex in Colombia sexed semen obtained through the technique of flow cytometry is used, this technique makes a separation of sperm through its genetic load being the X chromosome of greater genetic conformation than the chromosome Y; directly influencing the definition with more precision the sex of the animal, focusing its use on the needs of production. In a conventional straw, they can be found from 10-12 x 106 to approximately 20 x 106 sperm. In a sexed straw, the range is shorter, being approximately 2 x 106, so producers should use these straws in females whose reproductive characteristics are high or in first-time females, which are very fertile, due to their reproductive start. The expert technical support for the implementation of this tool is decisive, since it alleviates the shortcomings that may arise due to lack of personnel capacity and above all due to lack of knowledge. With this document, we intend to review the technique of insemination with sexed semen and its direct implications on livestock.