Background: Determine knowledge and attitudes towards promoting blood donation in Physiotherapy Program teachers at the University of Santander - Colombia. Materials and methods: We performed a cross-type study with teachers of physical therapy program at the University of Santander - Colombia in 2013. Results: The study population consisted of 21 teachers in the physical therapy program with an average age of 37.05 years, the majority of patients were female, teaching exercise time was 9.20 years and only 23.8 of teachers had donated blood at some time in their life, in relation to the promotion of donation on total classrooms of teachers considered important donation. Among the reasons not to include “Fear of drawing blood” and highlights reasons for donating advertising. Discussion: Teachers are important actors in the process of promoting donation in the student population.