Background: The frequency of ABO blood group and Rh factor is an important factor in relation to the needs of blood components in the population, identifying a routine in blood banks. Materials and methods: A retrospective, cross sectional, descriptive study was per formed, period from January to March 2012 with data provided by the Foundation from Colombia Hematological volunteer blood donors who attended a fixed point of blood collection in the city of Tunja - Colombia. The variables analyzed were age, gender, and Rh blood group ABO. Results: The study population consisted of volunteer donors 1678 the average age of 28,29 years, 53,3% (n = 894), 62,9% of the population belongs to the group O, there was higher proportion of positive versus negative Rh (94,9 vs 5,1 ), blood type O positive, 32% (n = 532) of the total population. Discussion: The group O and A are predominant in the study population, similar to behavior reported in the literature loa, just as the Rh negative.