Background: Parental acceptance of behavior guidance techniques is dynamic and influenced by social and cultural factors. Aim: To compare the degree of acceptance of behavior guidance techniques between American and Colombian parents. Design: American parents (n=150) and Colombian parents (n=150) of children between 3 and 12 years of age undergoing Pediatric Dental treatment participated in this multicenter cross-sectional study. Parents viewed a video depicting ten behavior guidance techniques approved by the American Academy of Pediatric Dentistry and rated their acceptance on a Visual Analogue Scale. Differences in the degree of acceptance were analyzed using quantile regression analysis. The level of significance was set at 5%. Results: Significant differences in the degree of acceptance of behavior guidance techniques were observed between American and Colombian parents (p <0.05); American parents were more accepting of protective stabilization, sedation, and general anesthesia, while Colombian parents were more accepting of communication techniques.