The present study shows the results of obtaining fermentable sugars using elephant grass (Pennisetum purpureum) feedstock. The concentration effect of acetic acid (CH3 COOH) and sodium chlorite (NaClO2 ) was assessed on the removal of lignin hemicellulose complex. A comparison was made about method, using basic pretreatment with sodium hydroxide (NaOH) and hydrogen peroxide (H2 O2 ). The using cellulase and β-glucosidase. The results show in the case of pretreatment with NaClO2 and CH3 pretreatment with a percentage 56.82% and hydrogen peroxide with a percentage of 44.75%