In the department of Cauca hand of the indigenous community of the Nasa Yuwe they have been strengthened and expanded knowledge that until some time ago were restricted, but now with a collective and cooperative work has been made publicly available and whom I interese.Tierradentro comprising the municipalities of Paez and Inza with an area of 2,764 km2, it has been one of the areas of interest at the time of the ethnobotanical research, and therefore in this place have developed characterization work, identification, management, among others (Peace & Betancourt Perez Delgado, NR) of plants used for food, traditional medicine, food, housing and economía.Este Bean is the case Cacha, a Fabaceae that has a high nutritional, cultural and socio-economic value among rural communities and Indians in the southwest of the country (Sanabria Diago, Guevara Rosas, Navia Imbachi, Orjuela & Muñoz, 2009), but which still lacks study many of their biological properties, among which we could place its antibacterial activity. Therefore, this work had as general objective to determine the inhibitory effect of the extract of Phaseolus dumosus, obtained from the seed (seed coat and cotyledon) on the in vitro growth of gram positive bacteria (Staphylococcus aureus) and gram negative ( Escherichia coli). Importantly, the identification of the species was made during the investigation, from morphological characters presented by Sanabria Diago, Guevara Rosas, Navia Imbachi, & Orjuela Muñoz in 2009; reporting and individuals of the species mentioned above in the town of Belalcazar, Taravira.Además path was made a preliminary phytochemical study of the testa and cotyledon of beans that allowed analyzing the major groups of secondary metabolites present in the plant. To head the representative group of the quinones it was followed by flavonoids; while for the cotyledon steroids, quinones, and cardiac glycosides were very representative. In story to the determination of the inhibitory effect on bean seed (testa and cotyledon) standardization and final testing they were conducted according to the methodology proposed by Rios, Recio and Villar in 1988, where the antibacterial activity is evaluated by the produced halos around 20μL of sample wells at different concentrations. This allowed report sensitivity of the extracts separately as halos in the case of the head 20 is generated between - 28 mm and in the case of cotyledon between 15 and 18mm.
