Bats of the genus Eumops (Chiroptera, Molossidae) are represented by 17 Neotropical species. In Colombia, there are limited records of eight species of Eumops in both cis- and trans-Andean localities and morphometric information is scarce. To explore the richness, morphology, evaluate the phylogenetic position and update the distribution of Eumops from Colombia, specimens deposited in biological collections were reviwed, and explore morphometric differentiation, obtained cytochrome-b sequences (cyt-b) of three species, and added new records based on specimens obtained in the field. Nine species distributed in Colombia were documented, morphological traits were used for identification of the species and confirm the presence of E. auripendulus, E. glaucinus, E. hansae, E. nanus, E. trumbulli and E. perotis. The E. glaucinus specimens from trans-Andean regions are morphometrically distinct from their cis-Andean conspecifics and other similar species distributed in Central and North America (e.g., Eumops ferox). However, genetic information is lacking to determine if these trans-Andean specimens correspond to either of the previously mentioned taxa. Populations from the eastern plains of Colombia, which have been morphologically associated with E. nanus, are genetically related to E. bonariensis. It is possible that E. nanus is distributed in trans-Andean localities of Colombia, whereas the cis-Andean records correspond either to an extension of the distribution of E. bonariensis or to a new species of small-sized Eumops in eastern Colombia. This, along with the scarce information on the presence of E. delticus and E. dabbenei in the country, highlights the limited knowledge about Eumops in Colombia and underscores the need for integrative studies to clarify the species richness and distribution within the region.