Human teeth biobank (HTB) is a higher education linked non-profit entity that collects, stores and administers donated teeth. Such an entity is supported by sanitary, biosafety, ethical and legal regulations. The purpose of this article is to describe the creation and development of an HTB comprising the biological, ethical, legal and biosafety aspects and the importance of keeping extracted teeth under controlled, reproducible and standardized conditions that do not affect their structure and properties for specific academic and research uses. For the creation of this HTB in the School of Dentistry of the Universidad Nacional de Colombia at Bogotá, this process started with a literature search and analysis after which ethical and national health laws were adopted following Colombian legislation.