The Euophryini tribe is currently one of the most diverse groups of salticidae. Within this group, the neotropic is represented by 11 clades, included the Antillattus clade (Antillattus 13 spp, Truncattus 5 spp, Petemathis 5 spp, possibly Allodecta 1 spp and Caribatus 1 spp). To clarify the phylogeny and biogeography of the Antillattus clade, we amplified and sequenced three genes (nuclear: 28S rDNA; mitochondrial: 16S, COI) corresponding to species belonging to the study group and outgroups (68 terminals). In addition, a total of 125 morphological characters were used, which in combination with the molecular evidence, helped to clarify the relationships between genera and species. Additionally, the GAARlandia hypothesis and the non-GAARlandia hypothesis are tested as possible routes of colonization and diversification of the Antillattus clade. The combined working hypothesis (DNA + morphology) supports the monophyly of the Antillattus clade. The results indicate that the genus Antillattus sensus Zhang and Maddison (2015), is not monophyletic, and it is divided into the genus Pensacolatus, Antillattus and Bryanattus gen. nov.. The results also supported the transferences of species to the genera Truncattus, Bryanattus gen. nov., Cobanus, Compsodecta, and the description of the genus Paracobanus gen. nov.. The detailed review provides new limits of genera and species, 19 comb. nov., 2 gen. nov., 11 sp. nov.. Finally, the results also suggest that the radiation of the group occurred in the last period of GAARlandia and that diversification within the Greate Antilles is the result of vicariance and founder-event. Additionally, evidence suggest that Hispaniola played a role as a point of dispersion to other Antillean islands.