Introduction: White spot lesion (WSP) is still one of the major problems associated to orthodontic treatment due to the high retention of dental plaque over the complex geometry appliances that make difficult the maintenance of a good oral hygiene. One strategy to avoid this problem is to modify the materials surface with antiadhesive molecules such as ethylene glycol. Methods: This study aimed to modify the surface of stainless steel 316L, commonly used in orthodontic appliances, with self-assembled monolayer of ethylene glycol (OEO-SAMs) using concentrations of 0,5 and 1 Mm, with and without thermal treatment (120 °C) to stabilize the bound between the surface and the monolayer. Surface characterization was made using the water contact angle measurements and then bacterial adhesion was evaluated with S. mutans. Results: The contact angle of SAMs with thermal treatment was equal to the stainless steel (indicating that probably the SAMs were not formed) while SAMs without thermal treatment showed lower contact angle than stainless steel (indicating their formation). On the other hand, microbiologic assays have showed an increase in bacterial adhesion, revealing that the monolayer was incomplete or not well formed. Conclusions: In this work, SAMs of ethylene glycol were prepared and preliminary characterized by contact angle measurements, which showed their formation. However, these OEO-SAMs did not allow reducing the adhesion of the cariogenic S. mutans. Therefore, complementary studies are still needed in order to decrease bacterial adhesion onto SS.