The increase in resistance to antibiotics, constitutes a threat to human health and one of the biggest concerns in the world. For this reason, since 2001, the Group for the Control of Antimicrobial Resistance in Bogota (GREBO) has implemented a surveillance system according to the World Health Organization. Objective. To determine the antimicrobial resistance profile of bacteria isolated from pediatric patients in the hospital and outpatient settings of public and private institutions of the city of Bogotá D.C., from January 2010 to December 2015. Methodology. A retrospective descriptive observational study was performed. We included 61,347 bacterial isolates of pediatric patients from the hospital, pediatric ICU, neonatal ICU and outpatient clinics of ten public and private institutions in the city of Bogotá. We studied secondary source variables related to demographic and clinical aspects of patients, as well as those related to clinical laboratory diagnosis. The antibiotics to be analyzed were defined according to the established clinical protocols and the corresponding antibacterial spectrum. Stata 12.0 © was used for the statistical analysis and frequency, central tendency and dispersion measures were used. Results. It was evidenced an increase of E.coli resistance from the year 2010 to 2015. Antibiotics such as ampicillin Sulbactam, Cefotaxime, Cefepime, Ertapenem have lost sensitivity to this germ. Imipenem, Meropenem and Amikacin maintained good sensitivity patterns for S. aureus and there was no significant decrease in sensitivity to oxacillin. Conclusion. Socializing the interior of health institutions, the results of surveillance systems in antibiotic resistance, ensure adequate treatments and improve the resistance patterns of some bacteria to antibiotics.