Objective: the goal of this study is to compare the effects on tooth enamel by using three stripping methods. Materials and methods: experimental in-vitro study. Twenty premolars were analyzed, divided in four groups, one control group and other three experimental groups in which stripping was made using diamond-coated metal strips, fine diamond bur, and fine diamond disks. Surface features were assessed using scanning electron microscopy (SEM), roughness test (Ra) and micro-hardness test (Hardness Vickers, HV). Results: differences among values from three stripping methods weren’t statistically significant. Average reduction to these three methods was 0.38 mm by tooth, being greater with disc and bur; otherwise SEM reported greater surface irregularity in group of diamond-coated metal strip. For roughness test the surfaces using fine diamond bur reported greater roughness average (Ra= 0.54 ± 0.20), a value statistically significant compared to control group (p= 0.001). For micro-hardness test the surfaces with greater values were those ones using diamond bur (365 ± 26.9). Conclusions: all the worked surfaces were morphologically altered and an increased roughness, especially the stripping applied with fine diamond bur and diamond-coated metal strip.