Cytoplasmic Citrus leprosis virus (CiLV-C) is one of the causal agents of citrus leprosis disease (CiLD) in South and Central America. CiLV-C was reported from Colombia in 2006. In 2011, Valencia and Navel orange trees affected with CiLD from La Libertad and Villavicencio, Colombia were sent to the USDA-APHIS-PPQCPHST, Beltsville, MD, USA for testing. Antisera against known CiLV-C failed to react with CiLD samples from these areas in ELISA tests. In addition, CiLV-C RTPCR specific primers also failed to produce amplicons