This chapter aims to discuss the microbiological analysis of biofilms associated with stone monuments in Villa de Leyva and the evaluation of biocides for their control. The role of microorganisms in the deterioration of historic monuments is related to climatic conditions and to the physical and chemical properties of construction materials. Villa de Leyva, Boyaca, considered as Colombian archeological and architectonic heritage, is characterized by a dry climate, and features numerous stone monuments composed of quartz and
