Biological control of Trialeurodes vaporariorum (Homoptera Aleyrodidae) by Amitus fuscipennis (Hymenoptera Platygastridae) with or without Encarsia formosa (Hymenoptera Aphelinidae) was tested in both a glasshouse and a plastic greenhouse during two consecutive production cycles of a beef tomato crop on the Bogotá Plateau in Colombia. The mean temperature was around 16° C in the plastic greenhouse and around 17° C in the glasshouse. A. fuscipennis was introduced at a rate of 5 pupae per m2 per week during the