La Doctrina district makes a great contribution to Colombian economy with production of several crops such as rice (1230 ha), corn (50 ha), African palm (300 ha), papaya (10 ha), vegetables (8 ha), and pastures (400 ha). To control pests in the crops farmers use organochlorine and organophosphate pesticides in irrigation channels, contaminating drinking water. This work sought to determine the concentrations of organochlorine (POCls) and organophosphate (OP) pesticide and cypermethrine (CP) in irrigation channels of La