Materials and methods: Information concerning 84 patients with epidermal growth factor receptor (EGFR) mutations was taken to explore Klotho's cytoplasmic positivity using an anti-Klotho antibody (Calbiochem, BD Biosciences, San Jose, CA, USA; 1: 100 dilution) and RT-PCR. The results were correlated with multiple outcomes, including differing clinical characteristics, response rate, progression-free survival (PFS) and overall survival (OS). Results: Mean age was 60.7 years (SD±13.1) and Klotho expression in the population of
