PYVV, family Closteroviridae, genus Crinivirus, is a phloem limited virus with a tripartite, ssRNA genome, covered with a two protein capsid, found in Andean countries. Symptoms include yellowing of the leaf apex until leaflets are fully cholorotic. PYVV is vegetatively transmitted and its vector is Trialeurodes vaporariorum. In this work, PYVV was detected by RT-PCR (retrotranscriptase PCR) in leaves and shoot-tubers from symptomatic Solanum phureja (egg yolk) plants. ds RNA was used as template for RT-PCR, using primers for part
