BACKGROUND: Central line-associated bloodstream infections (CLABSI) related to insertion and device care in intensive care units are frequent and preventable events. AIM: To evaluate the reduction in the rate of CLABSI through implementation of an insertion bundle. METHODS: A study was conducted in the Adult-ICU at the University Hospital of Neiva comparing a pre-interventional period with an interventional one, each lasting 6 months; the intervention consisting of implementing a bundle of measures for the insertion of