ABSTRACT: In this study, four microsatellites (Pme4, Pme5, Pme20, Pme21) were used to typify thirty individuals of Piaractus brachypomus from the Meta river, tributary of the Orinoco river basin. Results showed an average of four alleles per locus, six new alleles (Pme5202, Pme5204, Pme20201, Pme20211, Pme20217 y Pme21258), no reported in the reference species Piaractus mesopotamicus; the expected heterozygosity average value was 46.8% and the observed was 54.2%. Individuals were not affected by population genetic phenomena such as individuals entry or exit, mutation or artificial selection. These microsatellites could be used to the genetic analysis in this species in both conservation programs and brood stock selection in culture zones.