Three Aspergillus species; A. japonicus 246A, A. tamarii 3 and Aspergillus sp. GM4, were previously selected and had their ability to produce tannase tested in Adams, Czapeck, Khanna, M5 and Vogel culture medium for enzyme production. Aspergillus sp. GM4 and Adams medium were selected to perform this study. Tannase production was tested with different inducers. The highest induction ratio was in the presence of 2% tannic acid and 1% methyl gallate. The Plackett-Burman screening design was used and MgSO4 and agitation rate were selected for the complete factorial. The Central Composite Rotatable Design allows an increase of 2.66-fold in the enzyme production and reduced the costs of the medium production. The purification process of tannase from Aspergillus sp. GM4 was performed with filtration using 100 kDa molecular mass cut-off membrane and gel-filtration chromatography using Sephacryl S-200. The results showed 29% enzyme yield and 29-fold purification in the first step, while in the second step 3.5% enzyme yield and 33-fold purification was obtained. The native molecular mass was estimated by gelfiltration chromatography and resulted in a protein molecular mass of approximately 162 kDa. Aspergillus sp. GM4 tannase presented optimum temperature at 40°C and optimum pH at 6.0. The enzyme retained activity in pH 3.0-8.0 and the thermostability assay showed enzyme activity up to 80 °C, but after 30 min it was inactivated. Tannase was stable in the presence of compounds traditionally reported as inhibitors of the enzyme activity, such as βMercaptoethanol, EDTA and different metal ions, such as Pb and Ba.