The maturity stage is considered a factor decisive in the marketing of horticultural products, mainly because its direct related with the quality and postharvest shelf life. Castilla blackberry (Rubus glaucus Benth) is considered a non-climateric highly perishable fruit, presented in its harvest heterogeneity, in shape, size, color, weight and composition, generating high losses during the harvest, post-harvest and marketing that in consequence affect the market stock and economic losses. Therefore, the aim of this study was establish the proper harvest maturity for marketing of blackberry from Canyon Combeima, which was assement of physical, chemical and physiological with maturity level 4, 5 and 6 according to standard Icontec 4106. The results showed significant differences in maturity stage 4 respect to fresh weight was reduced 12 %, minimum soluble solids content (6.93 ± 0.12 oBx) influencing maturity index and higher respiratory rate during refrigerated storage, both of which reduce the quality and shelf life of this fruit. It was concluded that the state of maturity harvest and marketing recommended of the product evaluated is five, considering that in that state it reached adequate accumulation of acids, soluble solids, size and characteristic form of the fruit, without significant differences in weight when compared with the actual commercial maturity (GM 6).