ABSTRACT: To identify and describe the perceptions of nursing researchers, educators, and graduate students regarding the barriers to, and facilitators for, EBN in Medellín, Colombia.Methodology. Using a qualitative descriptive design, in-depth-semi-structured interviews were conducted with 12 participants associated with a large university faculty of nursing in Medellín,and one member of the National Association of Nurses. Qualitative content analysis was used to analyze the interview transcripts.Results. Several barriers to EBN were reported, including: lack off recognition of nursing as an autonomous profession; a lack off incentives for nurses to pursue advanced education or engage in research; limited availability and utility of nursing evidence;and a lack of communication between academic and clinical practice environments. Perceived facilitators included an increase in nurses pursuing advanced education opportunities; the current healthcare accreditation process; access to international research and research collaborations; and clinical and research partnerships between universities and clinical institutions.Conclusion. Effective implementation of evidence-based nursing practices is a necessity to translate the vast amount of health related research, knowledge, and experience into positive changes in healthcare quality.Key words: evidence-based nursing; education, nursing; research,nursing; information dissemination.