In the present research work was carried out the characterization and evaluation of commercial and experimental chitosan. For The latter isolation, growth, multiplication and macro and microscopic spores of the fungus Aspergillus niger was carried out initially identification, then extract the chitosan of the cell walls, there or by highly acidic and basic processes. The deacetylation degree of 93.5 and 92.5% was obtained for the experimental and commercial chitosan respectively. Both chitosan were characterized by thermal and chemical analysis and the carried out the forming process by electrospinning method. It was possible to obtain films only with commercial chitosan, consisting of continuous fibers with diameters less than 1 micron, which generated a highly porous three–dimensional membrane that could be used as a scaffold for tissue growth.