In recent years, the plastic packaging industry has been investigating how to replace non-biodegradable petroleum-based polymers with environmentally friendly alternatives. In this sense, starch-based edible biofilms and coatings are becoming interesting options for food packaging because they are renewable, biodegradable, nontoxic, and economically cost-effective. Different research shows unconventional starch sources have the potential to provide new characteristics and are viable alternatives for the production of bioplastics. Furthermore, the addition of extracts or essential oils from plant material to starch films can improve their antioxidant, antibacterial, and barrier properties, such as permeability to oxygen, UV rays, and water vapor, can reduce the reproduction of microorganisms and prolong the shelf life of packaged foods; therefore, the objective of this work was to evaluate the physical, mechanical, and rheological properties of biofilms elaborated with mango kernel starch and hydroethanolic extract of mango peel. Mango kernel starch presented extraction yields of 44.05 ± 2.58 % with amylose values of 34.35 ± 0.46. Hydroethanolic extract of mango peel obtained by ultrasound-assisted extraction presents yields of 33.24 ± 2.48 %, total phenolic compounds with values 305.04 ± 10.70 mg GAE/g and antioxidant activity analyzed by ABTS assay with TEAC values of 1470.41 ± 59.75. Different biofilms were developed evaluating the percentage of extract (0.5%–2%). Their properties depend mainly on the starch properties, that is, the source, the ratio of amylose to amylopectin, the size of the granule will influence the physical, mechanical, and rheological properties of the films. In addition, mango peel extract imparts bioactive properties to films, such as improved antioxidant properties. This research provides the possibility of using mango by-products to develop bioactive films that can be successfully used in the food packaging industry.