Objective: To establish the kinematic characteristics of the cycling gesture of pedaling in indoor cycling instructors with respect to sociodemographic, anthropometric and technical variables. Methodology: The study was carried out with a total of 15 instructors selected at convenience, the evaluation protocol was applied characterized by the anthropometric measurement, recording of technical variables related to the practice, and application of the biomechanics test Using high-speed motion capture software and 3d reconstruction, to determine the kinematics of the sporting gesture. Results: The parameters of normality were found in relation to the angles of joint mobility for the inferior members in comparative form in the different planes of movement, determining the relation with the anthropometric characteristics and techniques of the Sports practice. It was determined the significant relationship between the height and recoil of the saddle with the movements made in the sagittal plane, presenting greater impact on the wider movements of pedaling associated with the displacement of the pedal. Conclusion: It was verified the little information available associated with the biomechanics of indoor cycling and the technical aspects derived, where the results obtained can facilitate the adaptation of the bicycle looking to maintain the biomechanical conditions of the Movement of the lower extremities based on the angles of normalcy found in the study. The data will serve as a basis for future research, which determines the relationship between the kinetic and kinematic characteristics of the gesture of pedaling, facilitating the optimization of movement and better adaptations for health.