This study characterizes agrobiodiversity’s contribution to food sovereignty through the comparisonof agroecological, indigenous and agroindustrial farming systems in the Departmentof Santa Cruz, Bolivia. The study parameters include productivity, the percentage of the crops’harvest destined for self-consumption, and the diversity of species produced and incorporatedinto the family diet. The agroecological system shows to be the most biodiverse and considerablythe most productive (15 times greater than the agroindustrial farms studied); allocates asignificant portion of the yield to self-consumption (26% compared to 4% in the agroindustrial);and it is an important source of nutrients in terms of the number of species included in thediet of the producing families (18 compared to seven in the indigenous system and one in theagroindustrial). These data reveal the ability of agroecology to effectively support the achievementof food sovereignty and nutritional security.