Objective: This study aims to report an accurate set of osteometric data and identify morphological differences in the vertebral skeletal samples existing in the Amphitheatre of the Department of Human Morphology of the Faculty of Medicine of the National University of Colombia. Methodology: This is a descriptive study comprising the osteometry and morphological description of 164 dry vertebrae, by direct measurement with digital calibrator and manual gonimetry. Result: In total, the current study reports on 35 cervical vertebrae, 88 chest vertebrae and 41 lumbar vertebrae. Statistically significant differences were found for levels C3 to L5 in the Antero-Posterior and Medium-Lateral diameters of the vertebral body and for C3-T12 levels at the pedicular angle. Conclusion: The level and function of each vertebral region remains one of the main factors affecting the size and morphological characteristics of the different vertebral segments. For the establishment of standards and references, in addition to the morphometric and descriptive analyses of the sample, demographic information is required to establish the relationship between society and skeletal biology.