Background: In the last decades, the prevalence of infections by carbapenem resistant gram-negative bacilli has been increased. Objective: To determine types and frequency of the diferent carbape nemases in Klebsiella spp. and Pseudomonas aeruginosa , in six hospitals in Bogota-Colombia. Methods: Descriptive and observational study, in six hospitals in the city of Bogota, in the period from January 2017 to August 2018. PCR were performed for KPC, GES, VIM, NDM, IMP and OXA-48 genes, in carbapenem resistant Klebsiella spp. and P. aeruginosa . Results: 52 P. aeruginosa isolates amplified a carbapenemase gene, of which 39 (75%) were positive for KPC, 11 (21%) for VIM and two co-productions of KPC and VIM. Regarding Klebsiella spp. 165 strains amplified at least one carbapenemase gene, 98% expressed KPC and four isolates had co-productions of metallo- β-lactamases and KPC. Discussion: This study provides valuable information, such as the increased production of KPC in P. aeruginosa and the co-production of KPC plus metallo-β-lactamases, which has an implication both in treatment selection, isolation precautions and patient prognosis.