Introduction: ANGPTL-3 is a protein that regulates the activity of LPL and could have an important role in in lipid metabolism during pregnancy. Methods: This is a prospective cohort study aiming to measure serum Angiopoietin-Like 3 (ANGPTL3) levels in healthy and preeclamptic women during three periods of gestation and three months postpartum and to correlate them with biochemical, hormonal and anthropometric indices. This includes a group of healthy pregnant women (n = 52), followed for three periods of gestation: early, intermediate, late and 19 of these women were analyzed three months postpartum. Additionally, data of 21 pregnant women belonging to the same cohort who developed mild preeclampsia were selected and analyzed. Results: ANGPTL-3 levels in non-pregnant women were higher than women in the first trimester of healthy pregnancy (119.28 ng / mL vs 72.78 ng / mL, P <0.05). During healthy pregnancy, a statistically significant decrease in serum ANGPTL-3 was observed in the first trimester, with no significant change in the second trimester and with a statistically significant increase in the third trimester, but still lower than the ANGPTL-3 levels of non-pregnant patients, which returns to their baseline values after delivery. No significant differences were found in ANGPTL-3 levels between healthy pregnant women and pregnant women with mild pre-eclampsia in any of the trimesters. There was no consistent correlation of ANGPLT-3 with triglyceride levels in the groups of pregnant women, and there was a correlation of LDL-C levels throughout gestation with mild pre-eclampsia (P <0.05). Conclusion: The decrease in ANGPTL-3 levels, probably mediated by insulin sensitivity, frees LPL from the inhibition of ANGPTL-3 and it can be regulated by other hormones, placental and non-placental, in order to organize the gestation energy metabolism in its different phases.