With the objective to contribute the discovering of drugs to treat the disorders endogen androgen-dependent like prostate cancer (CP) and prostatic benign hyperplasia, which are public health problem worldwide and in Colombia because their high morbidity and mortality in mature men, in this investigation the in vivo activity of the lichen origin compounds sphaerophorin (1) and atraric acid (2) was evaluated. These compounds have been active in vitro on targets involved in the biosynthesis and action of endogen androgens. The compounds 1 y 2 were obtained from the lichens Bunodophoron melanocarpum and Stereocaulon strictum using phytochemical techniques. Their identity was confirmed by their spectroscopic RMN 1H and 13C data that was compared with the literature. The in vivo activity on the biosynthesis and action of endogen androgens was determined by Hershberger bioassay using male Wistar rats. The animals were castrated under anesthesia (Ketamine 75 mg/kg and xilacine 10 mg/kg) intraperitoneal ten days before the experiment. Then, the rats were distributed randomly in 5 groups of 7 individuals each one. Three groups were the controls: vegetal oil (AV) (200 µL, vehicle); testosterone propionate (TP) (0.4 mg/kg dissolved in the vehicle); and TP (0.4 mg/kg) + finasteride (F) (positive control) (1 mg/kg dissolved in 200 µL of vehicle) subcutaneously. The fourth and fifth groups had the test compounds (both 2 mg/kg dissolved in 200 µL of vehicle) + TP (0.4 mg/kg) that were administrated once daily for 10 days subcutaneously. The day after the last administration, the animals were sacrificed and their organs androgen dependents (prostate, seminal vesicles, bulbocavernosus muscle, Cowper glands and glans) and no androgen dependents (adrenal glands, kidneys, and liver) were obtained and weight. This data was expressed as mg of organ per 100 grams of corporal weight. Additionally, the prostates were analyzed semiquantitatively using histoscore to observe some characteristics that could be a possible antiandrogenic effect in that organ. Considering that 1 and 2 did not decrease significatively (p<0.05) the weight of the androgen-dependent organs, can be concluded the compounds did not have antiandrogenic effect in vivo at the evaluated dose. They also had no in vivo effect on the non-androgen-dependent organs showing no toxic effect preliminarily at short term. Histologically, the prostatic tissue treated with TP was hyperplasic (100%) meanwhile the TP + F had a lower hyperplasic proportion showing that this drug has a protective effect on hyperplasia inducted by this androgen. The tissue treated with TP + 1 or TP + 2 also showed a lower proportion of the hyperplasic characteristics (40 and 60% respectively) showing a protective effect in vivo. The sphaeorphorin and the atraric acid are candidates to continue deeper studies that let develop them as drugs to the possible treatment of androgendependent diseases.