This work depicts the results of the study of the seedlings originated out of the Germination Seeds Bank (GSB) in two succeeding states of a sub-Andean forest, located in the biological reservoir Cachalu. One of these, located at 1.700 m.a.s.l (meters above sea level), is a mature forest where oak (Quercus humboltii Bonpland) is predominant, and the other is a late secondary forest located at 1.860 m.a.s.l. The work was carried out by a series of samples taken from each of the succeeding states throughout tour transects of 10 m of length, in which there were collected 12 soil samples, reaching a depth of 10 cm, each one of the samples with a volume of 3.981 cm3. For the evaluation of the GSB, the method of seeds germination under greenhouse conditions was used, and, following the results, the morphologic description, and the identification and evaluation of the density and abundance of seedlings obtained from soil samples was done; the results were confronted with field observations of different aspects related to the presence - absence of individual adults of the species that germinated from GSB. From the seeds bank of the two sites, 1.642 seedlings germinated overall, where 908 emerged from the samples of the GSB of the secondary forest, and 734 of the samples of the mature forest. The present work includes a rapid and a general visualization of the conceptual basis of the study, the detailed description and illustration of 14 species in state of seedlings, the results of the experiment, and the field observations.