Spinal cord injury is an unfortunate event that generally involves a condition that in the short, medium and long term has multiple implications not only for the person suffering from it but also for their family nucleus and society in general (DeVivo and Chen, 2011; Angeli et al., 2018). For its treatment with cell therapy, a variety of cells have been used, among which the transplantation of ensheathing glial cells (CGEO). Specifically in canines, CGEO of the olfactory bulb and olfactory mucosa have been used, obtained through moderately invasive surgical procedures. In this research we innovate in taking the sample endoscopically in the nasal cavity avoiding the surgical approach to the nasal sinus and we also innovate by performing the transplant with a percutaneous technique with ultrasound guidance for the introduction of the needle and the deposit of cells intramedullary level without the need for an invasive surgical procedure involving laminectomy or myelotomy. We use magnetic resonance imaging to identify the exact site of the injury to perform the transplant. We measured the effect of non-purified CGEO transplantation (containing an average of 45% CGEO) in a sample of 8 companion dogs that presented chronic traumatic spinal cord injury in the city of Bogotá. Neurological tests and gait evaluation were performed using the Olby scale in pre-transplantation and monthly post-transplantation for three months, finding significant changes in some of the variables analyzed. Therefore, we were able to conclude that the effect of CGEO transplantation in clinical cases of spinal cord injury in canines is safe and brought beneficial effects in some of the patients. It is worth clarifying that there were no recoveries in the general proprioception tests or in postural reactions, so we think that the observed improvements were possibly due to local changes in spinal circuits and not to a recovery of movement that depends on long ascending and long tracts descending.