The ultrasonic characterization methods are often used in different research fields (Biomedical and Industrial Applications) due to its significant low cost - high performance ratio, besides its advantages in the non-invasive and non-destructive acoustic assessment of fluids, materials and biological tissues. The ultrasonic characterization is based on the estimation of acoustic parameters from ultrasound backscattering signals procesing techniques, which allow conventional ultrasonic index measurements (as speed of sound (SoS) and the frequency dependent acoustic attenuation (?)) aiming to the construction of parametric images as a potential tools in the analysis of the microstructure of biological tissues in biomedical applications. This study evidence the advantages of the implementation of an ultrasonic system for the quantitative characterization as a method to support processing techniques of biological tissues.