Fusarium oxysporum (FOX) is one of the most difficult plant pathogens to control leading to important economic losses in several crops owing to Fusarium-wilt disease. Biological control through beneficial rhizobacteria has high potential to reduce the devastating effects caused by FOX. Bacillus amyloliquefaciens strain Bs006 has potential as plant growth promoter and biocontrol agent against cape gooseberry wilt disease. Although plant growth promoting phenomenon had been consistent in previous experiments, the biocontrol activity by Bs006 was variable, suggesting that biotic and abiotic factors in the rhizosphere affect the efficacy of Bs006. Thus, the main goal of this doctoral research was contribute to knowledge of Bs006-FOX-cape gooseberry-environment interactions. Results show that Bs006 synthesizes cyclic lipopeptides (CLPs) from the iturin, fengycin and surfactin families. However, incubation temperature and culture media affect the production of CLPs. Experimental evidence indicated that iturins have fungistatic effect on FOX, while fengycins have fungicidal effect in a concentration dependent manner. In gnotobiotic cultures, it was determined that Bs006 colonizes the cape gooseberry root surface, forming a biofilm and utilizes root exudates as a nutritional source. In this system, it was found that Bs006 synthesizes compounds of the three families listed above and is associated with the inhibition of FOX growth. In vivo studies indicated that biocontrol activity of Bs006 against Fusarium-wilt is influenced by the concentrations of the pathogenic inoculum, Bs006 inoculum and CLPs. These results have provided a framework in which to evaluate the optimal combination of Bs006 cells and supernatant/semi-purified extract or pure CLPs in future studies. Since the production of CLPs by Bs006 is lower in the rhizosphere than in artificial media and that propagules of FOX can be tolerant to CLP action, additional research is needed to design integrated management programs of this disease. These programs should include the development of a biopesticide based on Bs006 and information about compatibility between rhizobacteria, fungicides and another biological control agents.