Background: Bioceramic sealers have shown adequate properties when analyzed under standard conditions, only a few studies analyze the performance of the sealers with filling techniques involving heat. Purpose: To evaluate the penetration capability of BioRoot™ RCS (Septodont, USA) bioceramic sealer into the dentinal tubules, using it in three heat involved filling techniques: mixed-hybrid condensation (CHM), Guttacore, and vertical condensation (CV). Methods: An in vitro study with a total sample of seventy human single-rooted teeth divided according to the filling technique into three experimental groups (n = 10), two control groups (n = 10) and two standardization groups (n = 10), filled with BioRoot™ RCS marked with Rhodamine B. The teeth were sectioned at 1 and 5 mm from the apex, analyzed under confocal scanning laser microscopy and maximum penetration depth determined. A Tukey test (p <0.05) and a double generalized gamma statistical model were performed. Results: The highest tubular penetration was achieved in the CHM obturation group with statistical significance, followed by Guttacore, CV, single cone, and lateral compaction. No statistically significant differences were observed between the positive control and negative control groups. Conclusion: Penetration into dentinal tubules of BioRoot™ RCS sealer is greater in filling techniques that involve heat.