Spiders (Order Araneae) produce silk used for everyday tasks ranging from prey-capture and immobilization to reproduction and safety draglines. Spider silks are mainly composed of proteins called spidroins, which are members of the spidroin gene family. Spider silk is not only one of the toughest materials on earth, it is also a known non-immunogenic, flexible, and lightweight. Recently, silk specific transcripts (SSTs) were described for cob-web weavers (Theridiidae) increasing the interest in studying their role in silk composition and physical properties to allow the production of synthetic spider silk. We evaluated the presence of SSTs on three spider species: Acanthoscurria geniculata (Theraphosidae), Nephila clavipes (Araneidae) and Stegodyphus mimosarum (Eresidae) to investigate if these SSTs are conserved across families not closely related or if they are lineage specific. We found 148 SSTs to be present in A. geniculata, 220 in N. clavipes and 137 in S. mimosarum. Moreover, we found 121 SSTs to be present in all species. Most SSTs shared by all species are involved in molecular functions such as oxidoreductase activity, hydrolase activity and oxidation-reduction processes. Our results highlight the importance of not only spidroins but also of SSTs in spider silk production. We propose SSTs play an important role in silk production and their study can shed light into mechanisms to the production of synthetic silk with matching mechanical properties.