An observational epidemiological cross-sectional study was proposed, a relation to the exposure of Fungi in intramural environments and the presence of allergenic fungi nasal fossae was described. The study involved 126 workers from the Santiago de Cali University, who signed an informed consent and answered the adapted ISAAC survey (The International Study of Asthma and Allergies Childhood) to determine the prevalence of diagnosed and undiagnosed allergies. 71/126 (56.3%) worked block 1; 24/126 (19.1%) block 3 and 31/126 (24.6%) block 4, The in vivo Skin Prick Test (SPT) was performed on 75 workers. A prevalence of SPT + was found for Fusarium oxysporum of 10/75 (13.3%), Aspergillus fumigatus 5/75 (6.7%), Penicillium notatum 5/75 (6.7%), Alternaria alternata 5/75 ( 6.7%) and Cladosporium herbarum 1/75 (1.3%). This study allowed to establish a possible association between the presence of fungi the labor environment and the nostrils of workers with the positive response for the SPT test for Fusarium oxysporum.