Introduction: The emergence and spread of carbapenemase-producing Enterobacteria, in public health, is a serious threat to the health of patients and to public health (J.A. Reyes- Chacon, 2017). That is why laboratories are always searching for reliable, accurate methodologies with the best response opportunity in the search of both colonized patients and those infected by this type of microorganisms. Materials and methods: Retrospective observational study. Isolates of Enterobacteria with resistance to some carbapenemics, preservatives in the HUSI cemetery and positive BLEES Enterobacteria isolates, sensitive to carbapenems. Sensitivity, specificity, positive and negative predictive value were determined. The Kappa index was calculated. Results: Modified method of inactivation of carbapenemases (mCIM) obtained: 96% sensitivity, 98% specificity, 98% PPV, 95% NPV compared to GenXpert. The Hodge test obtained, Sensitivity, specificity, VPP, NPV 97% respectively. Four false negatives and false positives were detected with respect to the detection of EPC by mCIM. Two cases of false positives were detected with the Hodge Test. Conclusion: The possibility of these carbapenemase producing Enterobacteria is important to face this problem. The Hodge test continues to be the best phenotypic method for the confirmation of the presence of carbapenemases. The mCIM has an almost perfect match with Genexpert, generating a greater number of false positive results, it can be used as an alternative method.