Currently, food losses are generated due to the presence of spoilage microorganisms, therefore the use of active packaging that functions as a protective barrier is necessary. To produce this type of packaging, it has been recently proposed the use of ultrafine membranes incorporated with antimicrobial compounds that potentially serve as attachments in active packaging and favor the controlled release of the compound. Peptides are one of the most commonly incorporated antimicrobials, having a demonstrated antimicrobial activity against a wide spectrum of microorganisms. Meanwhile, the membranes can be made from biodegradable polymers by taking advantages of nanotechnologies, the electrospinning technique being of particular interest. Despite the advantages of the use of biopolymers for the manufacture of ultra-thin membranes as a devices for active packaging, they often have undesirable characteristics, especially low resistance to water, which compromises their structural stability for this type of application. An improvement alternative consists in the combination of polymers of different nature that improve the properties of interaction with water of ultra-thin membranes, without compromising their biodegradability and biocompatibility. In this study, ultrafine membranes were developed using electrospinning from a polymer mixture for the incorporation of the palindromic peptide LfcinB(21-25)Pal, synthesized from bovine lactoferrin, which has been shown to have antimicrobial activity against viruses, bacteria, and fungi. Firstly, the feasibility of producing membranes of pullulan (PUL) a highly hydrophilic polysaccharide, polycaprolactone (PCL) a hydrophobic biodegradable polyester, and PCL mixtures with poorly water-soluble polysaccharides (modified starch of potato and β-glucan).The membranes were morphologically characterized by Scanning Electron Microscopy (SEM) to observe the orientation of the fiber, its diameter, and the presence of imperfections. The structural characteristics were evaluated by Differential Scanning Calorimetry (DSC) to determine fiber crystallinity. Chemical characteristics were evaluated by infrared spectroscopy (FTIR-ATR) to evaluate the presence of characteristic functional groups for each fiber. As a final point, the wettability was evaluated by measuring the contact angle. Multilayer membranes (PCL-PUL-PCL) have structural characteristics of cylindrical and smooth fibers, with an approximate diameter of 100 nm and thermal stability at XI temperatures between 200oC and 300oC. The FTIR spectra of the membranes confirmed electrospinning did not generate modifications in the structure of polymers. Based on the above, these membranes were chosen for peptide encapsulation, PUL was used as an encapsulation agent for the peptide and PCL was used to coat PUL since the highly hydrophobic character of PCL which maintained the integrity of the membrane in the presence of water. Subsequently, it was possible to encapsulate up to 65% of the LfcinB (21-25)Pal within the PUL fibers at a maximum load of 50 mg peptide/g of PUL, to then coat them with PCL. The membranes were characterized by structural, physical, and morphological properties. FTIR analysis showed that there were no chemical changes in polymers or peptide after electrospinning. The evaluation of antioxidant activity by DPPH showed that membranes at the highest load of the peptide possess an antiradical activity of 5.21 x 10-4mg ± 1.12 x 10-5 of gallic acid/mg membrane. Finally, in previous studies carried out by the research group, it was found that the Minimum Inhibitory Concentration of the peptide, when encapsulated in a polymer membrane, was 17 μM against a strain of Escherichia coli. The above suggests the potential application of these membranes be incorporated in active packaging that prolongs the shelf life of foodstuff through the controlled release of the antimicrobial peptide.