Enzyme activities were evaluated (urease, protease, acid phosphatase and alkaline phosphodiesterase, β-glucosidase and arylsulfatase) in bacterial consortia (Bacillus subtilis, Brevundimonas diminuta, Flavimonas oryzihabitants) from either soil with potato cropping under conventional management with the application of agrochemicals (PWA) or grassland soils without the use of agrochemicals (GNA) on farms of three municipalities (Tausa, Villapinzon and Zipaquira) in the department of Cundinamarca, Colombia. The type of land use and the location affected the tested enzymatic activities. In the municipality of Villapinzon urease, acid phosphatase, phosphodiesterase and β-glucosidase activity were higher in PWA than GNA by 89, 71, 67 and 75% respectively. The municipality of Zipaquira presented the same trend in urease, β-glucosidase and arylsulfatase with higher activity in PWA than GNA by 50, 71 and 68% respectively. Finally in Tausa, the same behavior was found for protease, acid phosphatase, alkaline phosphatase, phosphodiesterase and β-glucosidase with 55, 20, 75, 82 and 87% higher activity in PWA than GNA.