The pilot wastewater treatment plant (PWWTP) of the Environmental Microbiology Laboratory, which is responsible for reducing the pollution of non-domestic wastewater from the teaching activities of the Faculty of Sciences of the Pontifical Xavierian University; in combination with the Sensity reactor, an academic and functional project of the Faculty of Industrial Design, generate semisolid residues, such as used lignocellulose meshes and biomass of Chlorella sp., whose final disposal processes could lead to the pollution of natural resources; therefore, their management can be addressed through their use as raw materials for the production of new bioproducts, such as the production of biochar by pyrolytic conversion. With this in mind, the objective of this work was to produce a biochar as seed germination and planting substrate from biogenic biomass generated in a wastewater treatment plant and a phototrophic reactor using a co-pyrolysis process. Obtaining the biomass of Chlorella sp. for its use as raw material presents a challenge, as the cells tend to be in suspension and not to sediment due to their colloidal character. For this reason, a coagulant agent is necessary to promote their recovery. Different coagulants were evaluated to meet this purpose and the cationic coagulant had the highest percentage of recovery (90.2 %) at a concentration of 60 mg/L. The liquid coagulation residue resulting from the coagulation process was evaluated as a germination substrate of Lolium sp., Lactuca sativa y Raphanus sativus seeds, with values of 92 %, 96 % and 42 %, respectively. In addition, it was determined that this liquid residue does not generate high contamination if it is re-used as irrigation water in the agricultural field according to Resolution 1207 of 2014, since it obtained a pH of 7.85, and a concentration of nitrates and sulfates of 1 mg/L and 40 mg/L, respectively. The biochar produced from lignocellulose meshes, microalgae and pine bark as a filler material was used as an immobilization matrix for a consortium of beneficial bacteria. The biochar and co-inoculated biochar showed a pH of 5.08 and 6.03, a moisture of 3.7 % and 42 % and a CF percentage of 5.8 % and 5.5 %, respectively. The biochar obtained a yield of 63.6 % and a fixed carbon yield of 7.5 %. Both biochar were evaluated as an invitro germination substrate of ray grass (Lolium sp.), lettuce (Lactuca sativa), and radish (Raphanus sativus) seeds, but only ray grass seeds showed a higher percentage of germination compared to control, obtaining 100 % in both treatments. Subsequently, plant growth was evaluated at greenhouse scale for 22 days. After this period of time, it was determined that biochar treatment obtained values similar to the control; however, treatment with co-inoculated biochar produced a negative effect on ray grass, lettuce, and radish plants, as they decreased by 82 %, 16.6 % and 87 % in terms of dry weight, respectively; possibly due to the presence of a disrupting microorganism. The results of this study suggest that the number of plants per treatment needs to be increased. As well as the time of evaluation of the greenhouse growth test in order to monitor more broadly the application of biochar and biochar co-inoculated on the development of ray grass, lettuce, and radish plants.