Introduction: Enterococcus are Gram positive bacteria that are normally found in the gastrointestinal tract, oral cavity and genitourinary tract. Today, about 30 Enterococcus species have been recognized, being Enterococcus faecalis the most representative species (80-90% of the total Enterococcus). Enterococcus faecalis is one of the most relevant microorganisms in endodontic failure. It can be found both in planktonic state, and in biofilms. A bacterium in planktonic state refers to a single-celled organism floating in an aqueous medium; the biofilm corresponds to a structured community, composed of bacterial cells enclosed in a hydrated polymer matrix of its own synthesis and that is attached to a solid surface, creating a protective field, favoring its growth and allowing to survive in hostile environments. There are genes, such as Esp, Asa373, gelE, CylA and EfaA, that encode proteins through RNA, and these proteins are expressed as virulence factors by Enterococcus faecalis. Sometimes the persistence of the infection is due to the pathogenicity provided by these factors from the bacterium. These virulence factors in Enterococcus faecalis are gene-encoded proteins that can give you the ability to produce biofilms and increase their pathogenicity. It is said that the factors can act in specific stages of biofilm formation such as: surface conditioning, colonization of microorganisms in planktonic state, metabolism and growth of adhered microorganisms (formation of a protective and highly organizaed layer known as mature biofilm) and the separation of microorganism from the biofilm colonizing other sites. Objective: Evaluate the expression of the virulence genes Esp, Asa 373, gelE, Cyl A and Efa A in Enterococcus faecalis strains of endodontic origin. Materials and Methods: Cross-sectional sampling study for convenience. 58 previously identified and isolated Enterococcus faecalis isolates were selected from patients who attended the endodontics clinic at the Pontificia Universidad Javeriana. To determine the expression of virulence factors, RNA extraction and RT-PCR were performed for the study genes in sessile and planktonic state. The presence of virulence genes was compared using Kruskal Wallis and the association of biofilm production with virulence expression was performed using Chi Square. Results: the expression in planktonic and sessile state respectively was: Esp 41,4% and 48,3%; Asa373 0% and 3,4%; gelE 37,9% and 46,6%; CylA 44,8% and 37,9%; EfaA 87,9% and 98,3%. The virulence factor most frequently in the planktonic and sessile state was the EfaA gene. Conclusion: the expression of the virulence genes Esp, Asa 373, gelE and EfaA in Enterococcus faecalis strains of endodontic origin was higher in sessile state; and only the expression of the CylA gene was higher in planktonic state. The only gene that had no expression in planktonic state was Asa373.