The aim of this study was to evaluate the effect of temperature changes during cryopreservation process on the sperm head morphometry in the goat semen. Fifty six ejaculates of 7 male goats of recognized fertility were evaluated by using the Sperm Morphology Analysis (ASMA) to determine the sperm head size and shape: Length (L), Width (W), Area (A), Perimeter (P), Ellipticity (E), Rugosity (R), Elongation (El), and Regularity (RE). FASTCLUS procedure was done to classify the spermatozoa (SPZ) of the data set into a reduced number of subpopulations (SP). Fresh: Subpopulation 1 (SP1) consisted of long and wide spermatozoa (35.12%), SP2 with SPZ of lower ellipticity (36.81%), SP3 in SPZ of average measurements (9.58%) and SP4 in small (18.48%). Cooling: SP1 (32.93%) are SPZ long, SP4 (18.54%) small, SP3 (12.88%) are similar to SP1, SP2 (35.66%) SPZ not hydrodynamic (very wide). Post thawing: SP2 (35.66%) wide SPZ, SP1 (30.92%) long and elliptical (hydrodynamic), SP3 (14.43%) similar to SP1, SP4 (21.53%) they are small cells. The minimal effects of the sperm cryopreservation process on the morphometry in goat semen were observed in the frequency of SPZ distribution in SPs and the characteristics of their forms for each subpopulation; the structure of the SP was maintained after the period of refrigeration and cold storage. In conclusion, cryopreservation did not modify specific parameters such as SPZ distribution within SPs (less than 5% of changes).
Tópico:
Sperm and Testicular Function
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FuenteRevista Cientifica-facultad De Ciencias Veterinarias