Thrips are considered insect pests that cause great economic losses in avocado, causing deformity in fruits, making them unfeasible for export. Some species of thrips are considered quarantine pests in the United States. On the other hand, the bacterial communities present in insects are known to play an important role in many crucial aspects of the life of their hosts, such as fitness, nutrition, development, protection against pathogens and survival in hostile environments. . In general, little is known about avocado thrips and the microbiota present in them. Therefore, the objective of this thesis was to study the molecular phylogeny and associated microbiota of thrips species (Thysanoptera: Thripidae), in commercial avocado (Persea americana Mill.) crops from eastern Antioquia. The thrips species present in the avocado were identified by morphological and molecular analysis based on partial sequences of data obtained from molecular DNA markers. The Cytochrome Oxidase I (COI) gene and the internal transcribed region (ITS). 2-parameter Kimura distances, haplotype networks and an approximation of the phylogeny of the thrips found in the avocado were performed with other sequences of thrips obtained from the sequence bank. 7 species of thrips are reported in avocado, Frankliniella gardeniae, F. gossypiana, F. panamensis, Microcephalothrips abdominalis, Thrips palmi, Scirtothrips hansoni and Liothrips perseae (Tubulifera). Two species of thrips T. simplex and T. trehernei were also found in the dandelion weed plant that is frequently found in avocado crops. The COI gene and the ITS region were useful tools to identify mostly thrips species except for F. gardeniae and F. gossypiana, which at the molecular level turned out to be the same genetic unit. Additionally, the microbiota present in avocado thrips in Antioquia was evaluated using dependent and independent culture methods. Bacterial isolates of individual and poles thrips were identified from macro and microscopic and molecular characterization by analysis of the inter-ribosomal region (ITS) and sequencing of the 16S RNAr and gyrase genes. For culture-independent methods Three thrips morphotypes were selected, DNA was extracted and microbial diversity present was analyzed by different molecular techniques, including amplification of the 16S rRNA gene region followed by time temperature gradient gel electrophoresis analysis (PCR-TTGE) and Next Generation Sequencing (NGS) using Miseq-Illumina technology. The culture-dependent results showed the presence of the Proteobacteria and Firmicutes phyla. The genera Bacillus, Serratia, Pantoea, Sphingomonas and Moraxella predominate. These results were corroborated by the results obtained by culture-independent methods. The TTGE revealed the presence of the phyla Proteobacteria, Firmicutes and Actinobacteria. Furthermore, a principal coordinate analysis showed differences in the microbiota between the three morphotypes of thrips: Scirtothrips hansoni (brown morphotype), Frankliniella (pale morphotype) y F. panamensis (dark morphotype). Likewise, a total of 641 unique OTUs were assigned, the richness showed to be greater in the brown morphotype, the data obtained suggest that there is small diversity of bacterial species in thrips species. The results indicated that the microbiota is composed of 6 phyla with a predominance of Proteobacteria (99%) followed by Cyanobacteria (37.81%), Firmicutes (7.48%), Deinococcus-Thermus (4.42%), Actinobacteria (3.23%) and Bacteroidetes (2.11 %). Significant bacterial genera were detected in the brown morphotype, such as the presence of the Wolbachia endosymbiont, and differences in the microbiota were observed between the morphotypes. In the brown morphotype associated with S. hansoni, Wolbachia prevailed (65-98%), for the pale morphotype of Frankliniella two genera Enterobacter (96.35%) and Rickettsia (46.04%) stood out, and in the dark morphotype of F. panamensis, Ehrlichia (96.13%) predominated. Principal Coordinate Analysis (PCoA) and Constrained Analysis of Principal coordinates (CAP) showed that the morphotype explains differences between the thrips microbiota 49.7% but did not show significant differences between the bacterial communities between the three morphotypes. (P> 0.05). On the other hand, in this investigation the natural infection and molecular identification of Wolbachia were detected in two morphotypes of natural thrips populations. The results showed 34.55% infection, and the presence of two Wolbachia supergroups A and B in thrips. Supergroup A present in the pale morphotype of Frankliniella and B in the brown morphotype of S. hansoni. The results suggest the presence of two new Wolbachia sequences in the two populations of thrips evaluated. In conclusion, these results allow us to elucidate the cultivable and non-cultivable microbiota of thrips, as well as the species of thrips present in avocado from eastern Antioquia. This will provide a baseline with information for future studies to clarify and complement the associated microorganisms, their interactions and the transmission dynamics of pathogens in the agronomic field and those bacteria with biotechnological activity.