Objective: Verify through an experiment if the producers’ level of confidence varies between different agrifood Supply Chains in the departments of Cundinamarca and Boyacá in Colombia. Methodology: An experiment based on Berg's trust game (1995) was designed to determine the levels of Confidence (quantities sent) and Reciprocity (quantities returned) of two samples of individuals whose main economic activity is the transformation of fruits and vegetables: 14 belonging to a long supply chain (in Boyacá) and 26 to a short supply chain (in Bogotá). Sample size was estimated using G*power. A survey and a trust game were applied. The game was double-blind, single-round, with complete information, equal initial endowments (8000 COP), multiplication factor 3, non-simulated counterpart and non-random payment. The results were processed in R software and descriptive statistics were analyzed. The assumptions for the application of parametric and non-parametric tests were checked. Through linear and Tobit regressions, possible descriptive variables of confidence and reciprocity were obtained, which were validated or refuted through the non-parametric Mann-Whitney-Wicolxon test (W) for two independent samples. Results: The quantities sent and returned by long supply chain producers were 4286 (standard deviation 2928) and 4857 (standard deviation 3024). The quantities sent and returned by short supply chain producers were 5231 (standard deviation 2088) and 6923 (standard deviation 4734). Positive offers were maintained by almost all participants. These results indicate positive Confidence and Reciprocity that broadly coincide with general measures of Confidence as DANE (2019), European Social Survey (2018) and The World Values Survey (2009). Conclusions: Confidence (W = 36 p-value 0.4601, α = 5%) and Reciprocity (W = 34 p-value 0.3767, α = 5%) are not different between the two samples. However, it is not rejected that Trust may be related to the type of housing (W = 64, p-value = 0.06628, α = 10%) and marital status (W = 11, p-value = 0.09548, α = 10%), and that Reciprocity may be related to the educational level (W = 21.5, p-value = 0.05894, α = 10%). Further research is recommended to establish causal relationships.